First order derivative spectroscopy method involves the measurement of absorbances at zero crossing point of other drug. A new, rapid, precise, accurate and sensitive first derivative spectrophotometric method is proposed for the simultaneous estimation of Omeprazole and Cinitapride Hydrogen Tartrate in combined dosage form. The drugs obeyed the Beer’s law in the concentration range of 1-20 ug/ml (r2 = 0.9996 and r2 = 0.999) for Omeprazole and Cinitapride Hydrogen Tartrate both. In the proposed method, absorbance was measured at 236.5 nm (Zero Crossing Point of Cinitapride) and 275 nm (Zero Crossing Point of Omeprazole). Accuracy of the method was determined by recovery studies and the average percentage recovery was found to be 98.55% and 100.44% for Omeprazole and Cinitapride respectively. The LODs for Omeprazole and Cinitapride Hydrogen Tartrate were found to be 0.298 μg/ml and 0.425 μg/ml & LOQs were found to be 0.881μg/ml and 1.259 μg/ml respectively. Statistical analysis proves that the method is selective and sensitive for estimation of Omeprazole and Cinitapride Hydrogen Tartrate in combined dosage form. The results were found to be within acceptance criteria according to ICH Q2 R1 guideline.
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